Carbon dioxide incubator control technology

Carbon dioxide incubator pollution-free control technology:

The carbon dioxide incubator simulates the formation of a similar cell/tissue growth environment in the living body such as a stable temperature (37 ° C), a stable CO 2 level (5%), and a constant pH (pH: 7.2) in the incubator. -7.4), a high relative humidity (95%), a device for in vitro culture of cells/tissues. Widely used in cell, tissue culture and culture of certain specific microorganisms, common in cell dynamics studies, breastfeeding Collection of animal cell secretions, carcinogenic or toxicological effects of various physical and chemical factors, research and production of antigens, production of antibodies by hybridoma cells, in vitro fertilization (IVF), stem cells, tissue engineering, drug screening, etc.

In living organisms, organisms have their own immune system to protect cells or tissues, but when cultured in vitro, there is no immune barrier to protect themselves. For the basic parameters of CO2 incubator temperature, CO2 and humidity, most incubators can meet the needs of research experiments. However, for the various pollution sources faced in the cultivation process, the control methods and effects of the various brands of dioxygen incubators are not the same, so the threat of large Zui in vitro culture is actually a pollution problem.

Major sources of contamination in carbon dioxide incubators: bacteria, fungi (mold and yeast), viruses, mycoplasma, non-homologous cells.

For cells, the ideal culture environment is also suitable for the survival of these pollutants. The incubator itself is not discernible, and most of the time in cell culture, the cells are located in the incubator, so whether the box can be inhibited Bacteria or sterilization is very important!

Bacteria: After bacterial contamination, the medium will change color in 1-2 days, which has obvious influence on cell growth. The contaminated cells should be quickly isolated from other cell lines, discarded after sterilization, and disinfected with laboratory disinfectants and culture dishes. Clean platform.

Virus: Due to the parasitic survival of the virus, it is isolated from the normal cells as soon as possible after the outbreak, and the disposal is relatively easy to handle, which poses a greater threat to the operator; although the virus-contaminated cells do not affect the primary culture, it is not safe to produce the vaccine. Therefore, the potential virus is a problem in the mass production of cells and the production of biological products such as vaccines and interferons.

Fungi (mold and yeast): Fungi grow slower, not as easy to detect as bacteria, but are contaminated once they are found; there are currently no good inhibitors, including the commonly used amphotericin, Once the pollution is easy to break out, especially the spores are hard to kill.

Mycoplasma: After mycoplasma contamination, because they do not cause cell death, they can coexist with cells for a long time. The medium generally does not turbid, the cells have no obvious changes, and the appearance gives a normal feeling. In fact, the cells have been affected by many aspects, such as causing Cell deformation, affecting DNA synthesis, and inhibiting cell growth are often overlooked by most experimenters.

Non-homologous cells: that is, cell cross-contamination, because the equipment and solutions required for each cell strain are not strictly separated during cell culture operation, one cell is often contaminated by another cell. At present, dozens of cells in the world have been contaminated by HeLa cells, causing many experiments to be invalid.

Therefore, once the above pollution occurs, the cells can only be discarded after the Zui, the experiment can not be saved, the experimental loss for many sampling difficulties can not be estimated!

The SANTN CO2 incubator is a representative of a high-quality incubator. In the non-polluting control, it uses a 90°C high-temperature hot and humid air sterilization technology, and the entire sterilization process is 25 hours, ensuring the complete elimination of all microbial sources. The more you come, the more favored by customers.

In the design of the cabinet, the SANTN carbon dioxide incubator has also been bacteriostatically designed from many aspects:

(1) The inner wall is made of polished stainless steel. Research shows that polished stainless steel can significantly reduce the growth of pollutants;

(2) The outer body is made of high quality stainless steel with an antibacterial coating;

(3) HEPA high-efficiency air filter design can effectively filter out potential sources of pollution and particulate impurities;

(4) Full arc, completely smooth inner liner structure, greatly reducing the pollution area; easy to clean and maintain;

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