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Carbon dioxide incubator concentration determination

December 05, 2023

How to control the CO2 gas concentration in the carbon dioxide incubator, the following will explain to you:
CO2 concentration control in carbon dioxide incubator is very similar to carbon dioxide temperature control:
1. CO2 concentration uniformity and temperature uniformity.
2. Concentration-controlled automatic calibration system and temperature-controlled temperature control system.
3. Concentration control is the difference between two sensors (infrared and thermal conductivity), while temperature control is the difference between temperature control methods (water jacket and air jacket).
Carbon dioxide incubator CO2 gas concentration control:
The carbon dioxide incubator gas is composed of a high concentration CO2 cylinder, a gas pump, a pressure regulating valve, a needle valve, a solenoid valve, a flow meter and a gas cylinder. (High concentration CO2 cylinders are provided by the user). When a certain concentration of high-concentration CO2 gas is input, the flow rate and time control of the regulating valve, the needle valve and the solenoid valve are controlled to ensure that a certain amount of CO2 gas enters the working chamber, and the CO2 concentration value in the automatic control working chamber is reached. This value is simultaneously displayed on the display circuit for easy observation. In order to facilitate the user to monitor and sample the concentration during use, a sampling and monitoring port is installed on the back of the CO2 box. Due to the adoption of the fast and slow double inflation method, the CO2 concentration in the working chamber can be quickly recovered and there is no overshoot after the CO2 box is opened and closed. During the operation of the CO2 tank, the working chamber is ventilated by the gas cylinder to maintain a stable CO2 concentration value.

How to determine the concentration of carbon dioxide in the carbon dioxide incubator

Abstract: The carbon dioxide incubator simulates the growth environment of a similar cell/tissue in the incubator. The incubator requires stable temperature (37 ° C), stable CO 2 level (5%), constant pH. (pH: 7.2-7.4), a higher relative saturation humidity (95%), a device for in vitro culture of cells/tissues.

Carbon dioxide incubator infrared sensor It is tested by an optical sensor to determine the level of CO2.

The IR system covers an infrared emitter and a sensor. When the CO2 in the cabinet draws on the local infrared emitted by the emitter, the sensor can check the amount of infrared radiation measured, and the amount of infrared light is used to correspond to the box. The level of CO2 in the body, so the liquid concentration of CO2 in the tank can be obtained.

Because the IR system confirms the concentration of CO2 liquid in the tank through infrared loss, and the particles in the box can reflect or partially borrow infrared light, the IR system is sensitive to some particles in the box, and the IR sensor has high efficiency air in the air inlet. The incubator of the shower is more suitable.

The office principle of carbon dioxide incubator thermal conductivity sensor to monitor the concentration of CO2 liquid is based on the survey of the thermal conductivity of the inner cavity air. The low thermal conductivity of the input CO2 gas will change the thermal conductivity of the air in the cavity, so that a The CO2 liquid concentration is directly proportional to the electrical signal.

One of the shortcomings of the TC tanning system is that changes in the temperature and relative humidity of the chamber can affect the sensor's very accurate accuracy.

When the door is opened more times, not only the concentration of CO2 liquid, but also the temperature and relative humidity will cause a great turbulence, thus affecting the precision of the TC sensor.

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